ABSTRACT
OBJECTIVE@#To explore new hallmarks affecting the prognosis of patients with laryngeal squamous cell carcinoma (LSCC) via investigating the expression of CyclinE and p27 in LSCC tissues.@*METHOD@#The expression of CyclinE and p27 was detected via Elivision immunohistochemical staining in 160 LSCC tissues and 20 normal laryngeal tissues (NLT). The relationship between CyclinE/ p27 and LSCC/ NLT was analyzed via Log-rank analysis. The relationship of CyclinE and p27 protein was statistically analyzed by spearman correlation analysis. The relationship between CyclinE/p27 and clinical-pathology-factors of patients with LSCC, such as age, gender, tumor site, diameter, differentiation, lymph node metastasis and PTNM stage were analyzed by Chi-square test. The relationship between clinical-pathology-factors, CyclinE, p27 and overall survival time of patients with LSCC was analyzed via Cox multiplicity and Kaplan-Meier survival analysis. A significant difference was recognized by P0.05 for all). A negative correlation was found between the expression of CyclinE and p27 protein, r= -0.767(P<0.05). Kaplan-Meier survival analysis showed that the overall survival rate of patients with LSCC was 36.9% (P<0.05). The 5-year survival rate in positive group of CyclinE was 8%, in negative group was 80% (P<0.05). On the contrary, the 5-year survival rate of patients with LSCC in positive group of p27 protein was 77.27%, the rate was 5.32% in negative group (P<0.05). Cox multivariate regression analysis revealed that lymph node metastasis, PTNM stage, CyclinE and p27 were independent risk factors of prognosis for patients with LSCC.@*CONCLUSION@#It is the molecular basis underlying the development and invasion/ metastasis of LSCC that activation of CyclinE gene accompanying inactivation of p27 gene. It is very important of co-detecting CyclinE and p27 protein to predict the prognosis of patients with LSCC.
Subject(s)
Female , Humans , Male , Middle Aged , Carcinoma, Squamous Cell , Metabolism , Pathology , Cyclin E , Metabolism , Cyclin-Dependent Kinase Inhibitor p27 , Metabolism , Head and Neck Neoplasms , Metabolism , Pathology , Laryngeal Mucosa , Metabolism , Pathology , Laryngeal Neoplasms , Metabolism , Pathology , Lymphatic Metastasis , Oncogene Proteins , Metabolism , Prognosis , Squamous Cell Carcinoma of Head and Neck , Survival RateABSTRACT
N-myc downstream-regulated gene 1 ( NDRG1 ), a member of the NDRG gene family, is a cell proliferation and differentiation related gene which usually express in various human tissues, especially higher in kidney, prostate and placenta. A variety of studies have reported that NDRG1 is correlated with cell proliferation and formation of periphery nerve sheath which might be regulated by hypoxia, anti-oncogene and metallic ion metabolism. Although NDRG1 gene expresses abnormally in tumor tissue, which is probably involved in tumorigenesis, development and metastasis, the exact role of NDRG1 in tumorigenesis is not clearly defined and further studies need to be done.